Investigating Filamentous Growth and Biofilm/Mat Formation in Budding Yeast: Figure 1.

An interrelationship between autophagy and filamentous growth in budding yeast.

Over the last 15 years, yeast pseudohyphal growth (PHG) has been the focus of intense research interest as a model of fungal pathogenicity. Specifically, PHG is a stress response wherein yeast cells deprived of nitrogen form filaments of elongated cells. Nitrogen limitation also induces autophagy, a ubiquitous eukaryotic stress response in which proteins are trafficked to the vacuole/lysosome f...

The plate-washing assay: a simple test for filamentous growth in budding yeast.

Filamentous growth is a foraging response that occurs in fungal species. It allows fungal pathogens to invade cells and tissues of a host organism. Budding yeast undergoes filamentous growth and can invade semisolid agar plates, penetrating the agar surface. These cells cannot be removed by rinsing with water and form an invasive scar. The plate-washing assay is an easy first test for filamento...

The regulation of filamentous growth in yeast.

Filamentous growth is a nutrient-regulated growth response that occurs in many fungal species. In pathogens, filamentous growth is critical for host-cell attachment, invasion into tissues, and virulence. The budding yeast Saccharomyces cerevisiae undergoes filamentous growth, which provides a genetically tractable system to study the molecular basis of the response. Filamentous growth is regula...

Figures and figure supplements Snf 1 / AMPK promotes the formation of Kog 1 / Raptor - bodies to increase the activation threshold of TORC 1 in budding yeast

Regulation of G2/M progression by the STE mitogen-activated protein kinase pathway in budding yeast filamentous growth.

Inoculation of diploid budding yeast onto nitrogen-poor agar media stimulates a MAPK pathway to promote filamentous growth. Characteristics of filamentous cells include a specific pattern of gene expression, elongated cell shape, polar budding pattern, persistent attachment to the mother cell, and a distinct cell cycle characterized by cell size control at G2/M. Although a requirement for MAPK ...